Coding

Part:BBa_K5048059:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-23)


HSP60


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 712
    Illegal SpeI site found at 1144
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 712
    Illegal SpeI site found at 1144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 712
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 712
    Illegal SpeI site found at 1144
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 712
    Illegal SpeI site found at 1144
    Illegal AgeI site found at 36
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 33


Design Notes

HSP60 is a protein expressed on the cell membrane of the human intestine epithelia and can bind to LAP.


Source

HSPD1 heat shock protein family D (Hsp60) member 1 [Homo sapiens (human)] - Gene - NCBI (nih.gov) CCD:CCDS Report for Consensus CDS (nih.gov)


References